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. 2001 Jun 11;153(6):1239–1250. doi: 10.1083/jcb.153.6.1239

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Bub1^K872R is kinase deficient. Bub1 protein in various extracts was analyzed by immunoblot (lanes 1–4), or immunoprecipitated for an in vitro autophosphorylation reaction (lanes 5–8). Bub1-depleted extract was supplemented with mock translation (lanes 1 and 5), Bub1 translation (lanes 2 and 6), or Bub1^K872R translation (lanes 3 and 7). Lanes 4 and 8, CSF-arrested extract. The migration of molecular weight standards is indicated on the left.