Figure 7.

Reversible suppression of cell contractility by BDM and Y-27632. SV-80 cells growing on the silicon rubber substrate and producing wrinkles (A and F) were treated with either 30 mM actomyosin ATPase inhibitor BDM (top) or 10 μM ROCK inhibitor Y-27632 (bottom). Images of BDM-treated cells were taken at 2.5 (B), 5 (C), and 9.5 min (D) after drug addition; the drug was washed off after 30 min incubation, and the last image (E) was taken at 45 min after washing. Images of the cell treated with Y-27632 were captured at 2.5 (G), 6 (H), and 9.5 min (I) after drug addition; the drug was washed off after 30 min incubation, and the image (J) was taken 50 min later. Bar, 20 μm.