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. 2001 Jun 11;153(6):1209–1226. doi: 10.1083/jcb.153.6.1209

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Chromosomal association of CeINCENP and kinetochore assembly are independent processes. (A) Wild-type or CeINCENP–depleted embryos were fixed and stained to visualize DNA (cyan), MTs (red), CeCENP-A, and CeINCENP. CeINCENP is depleted to levels undetectable by immunofluorescence. Nevertheless, CeCENP-A localizes to the condensed mitotic chromosomes. (B) CeINCENP-depleted embryos were fixed and stained to visualize DNA, MTs, CeINCENP, and CeBub1 (top) or CeMCAK (bottom). CeINCENP was depleted to undetectable levels as in A (not shown). The right panels in both rows show higher magnification views. Both CeBub1 and CeMCAK localize to the condensed paternal chromosomes in the absence of CeINCENP and form normal-looking kinetochore plates. Localization to the meiotically defective maternal mass is weak or absent for both markers. (C) A CeCENP-A–depleted embryo stained for DNA (cyan), MTs (red), CeCENP-A, and CeINCENP. CeCENP-A was depleted to undetectable levels, but CeINCENP still localized to chromosomes. Bars: (A) 5 μm; (B, left and middle) 5 μm; (B, right) 1 μm; (C) 5 μm.