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. 2001 Jun 11;153(6):1209–1226. doi: 10.1083/jcb.153.6.1209

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Both CeMCAK and CeBub1 require CeCENP-C to target to chromosomes. Wild-type or CeCENP-C–depleted embryos were fixed and stained to simultaneously visualize DNA (cyan), MTs (red), CeCENP-C, and either CeBub1 (A) or CeMCAK (B). CeCENP-C was depleted to undetectable levels (not shown). Centrosomal localization of CeMCAK and nuclear accumulation of CeBub1 occur in CeCENP-C–depleted embryos. However, neither protein localizes to chromosomes during any mitotic stage. Identical results were obtained in analysis of CeBub1 and CeMCAK localization in CeCENP-A–depleted embryos (not shown). All images are projections of three-dimensional stacks. Bar, 5 μm.