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. 2001 Jun 11;153(6):1209–1226. doi: 10.1083/jcb.153.6.1209

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Mechanically stable spindles do not assemble in embryos depleted of CeCENP-A or CeCENP-C. (A) Time-aligned stills summarizing spinning disk confocal videos of GFP–α-tubulin expressing wild-type (left) and CeCENP-C–depleted (right) embryos. The time, in seconds after NEBD, appears in the top right hand corner of each panel. In the absence of CeCENP-C, MTs invade the nuclear space after NEBD, but a stable spindle fails to form and the spindle poles move apart abruptly. Identical results are obtained in CeCENP-A–depleted embryos (not shown here). (B) Pole separation in wild-type (diamonds) and CeCENP-C–depleted (squares) embryos. Three sequences are plotted for each condition. The grey arrowhead indicates the average time after NEBD when the spindle initiates posterior movement in wild-type. The black arrowhead indicates the average time of anaphase onset in wild-type. In CeCENP-C–depleted embryos, the poles separate abruptly during the interval marked by the arrowheads. (C) The maximum rate of pole separation was determined for each experimental condition. The mean ± SD from analysis of five videos per condition are plotted. The maximum pole separation rate is significantly different between wild-type and CeCENP-C/CeCENP-A depletions (P = <0.001 and P = <0.05, respectively, in a Student's t test), but is not significantly different between CeCENP-A and CeCENP-C depletions. Online supplemental videos are available at http://www.jcb.org/cgi/content/full/153/6/1209/DC1. Bar, 5 μm.