Figure 3.

Inhibition by T138067 of microtubule formation in vitro. (A) SDS/PAGE of the cushion fraction from a sedimentation assay. Polymerization reactions (40 μM tubulin) were kept on ice (lane 1), 37°C (lane 2), or 37°C in the presence of T138067 [lane 3 (1 μM), lane 4 (3 μM), and lane 5 (10 μM)], colchicine [lane 6 (1 μM), lane 7 (3 μM), and lane 8 (10 μM)], or paclitaxel [lane 9 (1 μM), lane 10 (3 μM), and lane 11 (10 μM)] for 10 min before analyses. (B) Turbidimetric assay. Graphical representation of changes in the OD at 340 nm over time (min) in the absence (no drug) or presence of the indicated compounds.