Figure 4.

T138067-induced collapse of the cytoskeleton and cell-cycle arrest. (A) Phase-contrast images of MCF7 cells after treatment with T138067. MCF7 cells were either incubated in the absence of drug (no drug) or treated for 9 hr with the indicated concentrations of T138067. Representative images were taken at ×100 magnification. (B) FACS analysis of nuclei isolated from MCF7 cells that were untreated (no drug) or treated for 24 hr with T138067 at the concentrations indicated for the corresponding panel in A. DNA content (2n = diploid; 4n = tetraploid) is indicated.