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. 2001 May 14;153(4):735–744. doi: 10.1083/jcb.153.4.735

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Minicircle hybridization (determined by FISH) as a function of DAPI fluorescence. Cells undergoing minicircle replication from Fig. 2 B (detected by TR-dUTP labeling) were each evaluated for intensity of both FISH and DAPI labeling. Of the 254 cells undergoing replication, 153 had an interpretable pattern of FISH. Each of these cells was then individually assessed to determine whether they had weak (•) or strong (□) minicircle fluorescence on the flagellar face of the kDNA disk. Values for fluorescence intensity are arbitrary. Bar, 1.0 μm.