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. 2001 May 14;153(4):663–676. doi: 10.1083/jcb.153.4.663

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© 2001 The Rockefeller University Press

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Sites of P-element insertions in polo ⁹ and polo ¹⁰ and relative expression levels of their gene products. (A) Schematic diagram of insertion sites in which P-elements are indicated by the filled red triangles above the linear map at positions −34 and −176 nucleotides upstream of the initiator ATG codon of the Polo protein. Two major starts for the initiation of transcription are indicated by the horizontal black triangles at positions −130 and −210 nucleotides. Exons are indicated by the open box and the first intron by the kinked line. (B) Western blots to compare the levels of Polo kinase and γ-tubulin in wild-type (wt), polo, polo ⁹, and polo ¹⁰ larval brains (see Materials and Methods).

Sites of P-element insertions in polo ⁹ and polo ¹⁰ and relative expression levels of their gene products. (A) Schematic diagram of insertion sites in which P-elements are indicated by the filled red triangles above the linear map at positions −34 and −176 nucleotides upstream of the initiator ATG codon of the Polo protein. Two major starts for the initiation of transcription are indicated by the horizontal black triangles at positions −130 and −210 nucleotides. Exons are indicated by the open box and the first intron by the kinked line. (B) Western blots to compare the levels of Polo kinase and γ-tubulin in wild-type (wt), polo, polo ⁹, and polo ¹⁰ larval brains (see Materials and Methods).