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. 2001 May 14;153(4):663–676. doi: 10.1083/jcb.153.4.663

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Distribution of centrosomal antigens in wild-type, polo ⁹, and polo ¹⁰ cells. In all cases, wild-type cells are shown in the left panels, polo ⁹ cells the middle panels, and polo ¹⁰ cells the right panels. Spindle microtubules revealed by immunostaining with the YL1/2 antibody are shown in green, DNA stained with propidium iodide in red, and the centrosomal antigen in blue. (A–C) Centrosomin (Cnn) is revealed using a rabbit antibody from Heuer et al. 1995. (D–F) γ-Tubulin was detected using the mouse monoclonal antibody GTU88 (Sigma-Aldrich). (G–I) The Asp was detected using the rabbit antibody Rb3133 (Saunders et al. 1997). (J–L) CP190 was detected using the rabbit antibody RB188 (Whitfield et al. 1988). Bar, 5 μm.