Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 May 14;153(4):663–676. doi: 10.1083/jcb.153.4.663

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Association of Bub1 and the 3F3/2 epitope with the mitotic apparatus in wild-type and polo mutant cells. In all panels, spindle microtubules are stained green and DNA stained red. Bub1 (A–F) or 3F3/2 (G–L) staining are shown in blue. (A and B) Wild-type cells at metaphase and anaphase, respectively. (C and D) polo ⁹ cells showing Bub1 staining with the rabbit antibody Rb666 (gift of C. Sunkel, University of Porto, Porto, Portugal) on the separated kinetochores. (E and F) Wild-type cells at metaphase and anaphase, respectively, stained with the 3F3/2 mouse monoclonal antibody (gift of G. Gorbsky, University of Oklahoma, Oklahoma City, Oklahoma). Note the presence of the 3F3/2 epitope at centrosomes. (G and H) polo ¹⁰ cells showing 3F3/2 staining on the separated kinetochores, and absent from the spindle poles. Bar, 5 μm.