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. 2001 May 14;153(4):663–676. doi: 10.1083/jcb.153.4.663

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cyclins A and B in wild-type and polo mutant cells. In all panels, spindle microtubules are green, DNA red, and cyclin blue in the merged images. The monochromatic image is of cyclin staining alone using either the antibody Rb270 to detect cyclin A or Rb271 to detect cyclin B (Whitfield et al. 1990). (A) Cyclin A in a wild-type cell at prometaphase. (B and C) Absence of cyclin A staining in polo ⁹ and polo ¹⁰ mutant cells respectively. (D) Cyclin B staining in a wild-type cell at metaphase. (E and F) Cyclin B staining of polo ⁹ and polo ¹⁰ cells, respectively. Bar, 5 μm.