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. 2001 May 14;153(4):785–794. doi: 10.1083/jcb.153.4.785

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© 2001 The Rockefeller University Press

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WAP–EPM transgenic mice have enlarged ductal lumina and altered C/EBPβ expression. (A) Schematic diagram of the transgene construct. N, NotI; H, HindIII. (B) PCR analysis of the integration of the transgene into genomic DNA and RT-PCR analysis of EPM transgene expression. As a control for RT-PCR, endogenous stromelysin 1 (SL-1) was analyzed in the same samples. Lower bands (asterisk) are unreacted primers that remained in control samples after RT-PCR. (C) Phenotypic appearance of the mammary gland (circled) from midpregnant (7 d) normal (a) and transgenic (b) mice. Whole-mount stained mammary gland of normal (c) and transgenic (d) mice. (D) Analysis of C/EBPβ gene products in mammary tissue of normal and transgenic mice using commercial anti-C/EBPβ antibody. CRM, cross-reactive material. Bars: (B) 5 μm; (C) 300 μm.

WAP–EPM transgenic mice have enlarged ductal lumina and altered C/EBPβ expression. (A) Schematic diagram of the transgene construct. N, NotI; H, HindIII. (B) PCR analysis of the integration of the transgene into genomic DNA and RT-PCR analysis of EPM transgene expression. As a control for RT-PCR, endogenous stromelysin 1 (SL-1) was analyzed in the same samples. Lower bands (asterisk) are unreacted primers that remained in control samples after RT-PCR. (C) Phenotypic appearance of the mammary gland (circled) from midpregnant (7 d) normal (a) and transgenic (b) mice. Whole-mount stained mammary gland of normal (c) and transgenic (d) mice. (D) Analysis of C/EBPβ gene products in mammary tissue of normal and transgenic mice using commercial anti-C/EBPβ antibody. CRM, cross-reactive material. Bars: (B) 5 μm; (C) 300 μm.