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. 1999 May 11;96(10):5704–5709. doi: 10.1073/pnas.96.10.5704

Figure 1.

Figure 1

K1 induces signal transduction in the absence of exogenous ligand and is phosphorylated on the tyrosines in the ITAM. (A) Schematic diagram of the constructs used. (B) Histograms of the fold activation of luciferase in DT-40 or Raji cells cotransfected with the indicated plasmid and a plasmid containing luciferase under the control of a triple NFAT site versus activity in cells transfected with empty vector. (C) Antiphosphotyrosine (4G10) antibody Western blot of cells transfected with the indicated plasmid, lysed, and immunoprecipitated with anti-Flag tag antibody (lanes 1–3). Lanes 4–6 are the same blot stripped and reprobed with the Flag antibody. (D) Anti-HA tag antibody Western blots of cells transfected with the indicated plasmids, lysed, and immunoprecipitated with anti-Flag tag antibody (lanes 1–3). The supernatants from the immunoprecitates also were blotted to show expression of HA-K1 or ΔC in all transfections (lanes 4–6).