Table 1.
Flow cytometry on sera from 18 patients
| Patient no. | Dose level | μg | %
positive cells
|
|||
|---|---|---|---|---|---|---|
| IgM
|
IgG
|
|||||
| Pre- | Post- | Pre- | Post- | |||
| 1 | 1 | 10 | 9.6 | 12.8 | 9.1 | 9.9 |
| 2 | 1 | 10 | 10.9 | 30.7 | 10.1 | 10.8 |
| 3 | 1 | 10 | 9.9 | 25.2 | 9.2 | 4.9 |
| 4 | 1 | 10 | 10.8 | 26.4 | 12.7 | 10.4 |
| 6 | 2 | 30 | 10.8 | 52.2 | 10.5 | 10.4 |
| 7 | 2 | 30 | 9.0 | 11.2 | 10.5 | 10.3 |
| 8 | 2 | 30 | 10.5 | 96.9 | 9.5 | 34.9 |
| 9 | 2 | 30 | 11.1 | 47.5 | 11.7 | 33.3 |
| 10 | 2 | 30 | 9.5 | 16.3 | 9.7 | 14.2 |
| 11 | 3 | 100 | 10.5 | 23.2 | 10.4 | 8.2 |
| 12 | 3 | 100 | 9.9 | 32.4 | 10.1 | 13.5 |
| 13 | 3 | 100 | 10.9 | 63.0 | 9.2 | 12.9 |
| 14 | 3 | 100 | 10.6 | 19.8 | 9.4 | 15.9 |
| 15 | 3 | 100 | 9.6 | 33.6 | 11.4 | 12.7 |
| 16 | 4 | 3 | 11.4 | 19.9 | 10.6 | 5.9 |
| 17 | 4 | 3 | 9.9 | 4.7 | 9.9 | 13.2 |
| 18 | 4 | 3 | 9.1 | 7.1 | 10.3 | 2.2 |
| 19 | 4 | 3 | 8.5 | 8.9 | 11.3 | 6.3 |
| MBr1 mAb | 64.7 | — | ||||
| VK-9 mAb | — | 62.1 | ||||
| Unstained | 0.9 | 1.7 | ||||
Patients’ pre- and post-treatment sera were incubated with MCF-7 cells and then with FITC-conjugated anti-human IgM or IgG antibody. The percent fluorescent cells incubated with pretreatment sera were gated to have values of approximately 10% and were compared with posttreatment values. Positive controls included anti-globo H mAb VK9. Patient 5 was taken out of the study because of overwhelming infection. Patient 20 was taken out because of rapid progression of disease.