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. 2000 Oct 16;151(2):401–412. doi: 10.1083/jcb.151.2.401

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Increased BrdU labeling and interfollicular K6 expression in ML.VS transgenic epidermis. BrdU incorporation analysis was used to compare the distribution of S phase nuclei. BrdU labeling (yellow) of transgenic skin (a) shows an increase in mitotic activity versus control (b). Note the additional labeled nuclei in the basal layer in transgenic epidermis. The sections were also double-labeled with K14 (red). Marked interfollicular staining with an antibody to K6 (FITC) is evident in transgenic epidermis (c) compared with control (d). The epidermis was counterstained with antibody to K14 (Texas red). Yellow indicates colocalization at K14 and K6. In both cases, hair follicles stain positive for K6. Note strong interfollicular expression of K6 in transgenic tail from mice at day 7 after birth in addition to the staining of cells in the outer root sheaths.