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. 2000 Oct 16;151(2):311–320. doi: 10.1083/jcb.151.2.311

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Transcriptional regulation of osteoblast genes in primary Src^+/+ and Src^−/− calvarial cells. Src^+/+ and Src^−/− primary osteoblasts were grown to confluence, then RNA was extracted. 1 μg RNA was reverse-transcribed and the equivalent of 0.1 μg was subjected to PCR using primer pairs and conditions as described in the legend to Table . Densitometric analysis was performed as described in the Materials and Methods, then the density ratio between the gene being analyzed and the constitutive gene GAPDH was computed. Similar results were observed in three independent experiments, with P < 0.05 for Src^−/− versus Src^+/+.