Figure 7.
wt p10 and D23A p10 binding to the nuclear envelopes of permeabilized cells in the presence of other nuclear transport factors. Binding of wt and mutant p10 to the nuclear envelope of digitonin-permeabilized HeLa cells was performed as described in the legend to Fig. 6. 0.25 μM (dimer) wt p10 or D23A p10 were added either alone (top), or with 20 μM RanGDP, 20 μM Kap-β1, or 20 μM untagged wt p10.