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Inhibition of RanGDP import by high concentrations of Kap-β1. p10-mediated nuclear import of FITC–RanGDP was measured in the presence of increasing concentrations of Kap-β1. All samples contained 1.5 μM FITC–RanGDP, 0.5 mM GTP, 2 mg/ml BSA, and Kap-β1 at the indicated concentrations. In addition, samples contained 1.25 μM (dimer) WT p10 (○) or D23A p10 (•). Import was for 15 min before washing and fixation.
