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. 2000 Oct 16;151(2):341–352. doi: 10.1083/jcb.151.2.341

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The primary phenotype resulting from loss of MGM1 function is fragmentation of mitochondrial reticuli. Mito-GFP was used to visualize mitochondria in wild-type (A and B), mgm1-5 (C and D), and Δmgm1 (I and J) cells grown overnight at 25°C to log phase, and after shifting to 37°C for 25 min (E and F) or 65 min (G and H). Quantification of mitochondrial morphology phenotypes at 37°C (K). Bars, 2 μm.

The primary phenotype resulting from loss of MGM1 function is fragmentation of mitochondrial reticuli. Mito-GFP was used to visualize mitochondria in wild-type (A and B), mgm1-5 (C and D), and Δmgm1 (I and J) cells grown overnight at 25°C to log phase, and after shifting to 37°C for 25 min (E and F) or 65 min (G and H). Quantification of mitochondrial morphology phenotypes at 37°C (K). Bars, 2 μm.