Figure 2.

Mitochondrial fusion is blocked in mgm1-5 cells during mating. Cells of opposite mating type were grown to log phase at 25°C, labeled with either mito-GFP or MitoTracker and mated at 25°C (E–H) or 37°C (A–D, I–P). Mitochondrial fusion was assessed by examining in merged mito-GFP and MitoTracker images of large-budded homozygous zygotes formed from wild-type (A–D), mgm1-5 (E–L), and fzo1-1 (M–P) cells. Bars, 2 μm.