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. 2000 Oct 16;151(2):341–352. doi: 10.1083/jcb.151.2.341

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Mgm1p is a mitochondrial intermembrane space protein peripherally associated with the inner membrane. Whole cell extracts of JSY836 and JSY2519 were prepared as described in Materials and Methods and analyzed by SDS-PAGE and Western blotting with indicated antibodies (A). Subcellular fractions of enriched intact mitochondria were isolated, treated with PK after mock-treatment (B), sonication (C), or hypoosmotic shock (D) and analyzed by SDS-PAGE and Western blotting as described. E, Intact mitochondria were treated with 0.1 M sodium carbonate (pH 10.5) as described and fractions were analyzed by SDS-PAGE and Western blotting.