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. 2000 Oct 16;151(2):289–296. doi: 10.1083/jcb.151.2.289

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TRAPP does not stimulate the uptake of [³⁵S]GTPγS onto Ypt32p or Sec4p. Equal amounts of His₆-Ypt32p (A), or His₆-Sec4p (B) and His₆-Ypt1p were incubated with [³⁵S]GTPγS at room temperature with IgG–Sepharose beads, containing TRAPP or lacking it (see Control) exactly as described above for His₆-Ypt1p. At the indicated periods of time, the radioactivity bound to protein was measured. Note that Sec4p has a high intrinsic exchange rate as previously reported (Kabcenell et al. 1990).

TRAPP does not stimulate the uptake of [³⁵S]GTPγS onto Ypt32p or Sec4p. Equal amounts of His₆-Ypt32p (A), or His₆-Sec4p (B) and His₆-Ypt1p were incubated with [³⁵S]GTPγS at room temperature with IgG–Sepharose beads, containing TRAPP or lacking it (see Control) exactly as described above for His₆-Ypt1p. At the indicated periods of time, the radioactivity bound to protein was measured. Note that Sec4p has a high intrinsic exchange rate as previously reported (Kabcenell et al. 1990).