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. 2000 Oct 16;151(2):439–452. doi: 10.1083/jcb.151.2.439

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Binding of Sec1p to SNARE complexes in sec mutant strains. (a) Coimmunoprecipitation of Sec1p and Sncp with HA-Sso2p. Wild-type and sec mutant strains expressing HA-Sso2p and an untagged wild-type control were grown at 25°C and shifted to 37°C for 10 min. Sec1p and Sncp coprecipitating with HA-Sso2p in anti-HA immunoprecipitates were observed by Western blotting. Note that the strains in this experiment were shifted to 37°C rather than to 38°C as in Fig. 2. This lower temperature is partially permissive for SNARE complex assembly in the sec8-6 mutant strain. (b) Coimmunoprecipitation of Ssop with myc-Sec1p and Sncp. Wild-type and sec mutant strains expressing myc-Sec1p and an untagged wild-type control were grown at 25°C and shifted to 37°C for 10 min. Ssop coprecipitating with myc-Sec1p in anti-myc immunoprecipitates and coimmunoprecipitating with Sncp in anti-Sncp precipitates was observed by Western blotting.