Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Oct 16;151(2):249–262. doi: 10.1083/jcb.151.2.249

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

PDGF-induced fibroblast migration is blocked by expression of mutants interfering with the Cdc42/N-WASP pathway. (A) PDGF-induced migration of GFP transiently transfected NIH-3T3 cells was assayed in collagen VI–coated 96-well microchambers and quantitated by densitometry of the spots. Cell migration is expressed as a migration index and calculated as the x-fold increase over the negative control (unstimulated cells). Figure shows the mean of five experiments; SD is indicated. (B) Percent migration (induced by 10 ng/ml PDGF) of p110CAAX- or p85α stable transfectants compared with the migration of control stable transfectants (considered 100%). Figure shows the mean of three experiments; SD is indicated. (C) Percent migration (induced by 100 ng/ml PDGF) of cells transfected with a vector encoding GFP plus the different cDNAs (indicated), compared with the migration of control cells transfected only with cDNA encoding GFP (considered 100%). Figure shows the mean of four experiments; SD is indicated.