Figure 3.

Basic amino acids at the COOH-terminal flanking region of the TMD are critical for targeting rTom20-GFP fusions to mitochondria. COS-7 cells were transfected with the indicated constructs in the expression vectors. The cells were costained with MitoTracker. Other conditions were the same as in Fig. 2. (A) Localization of rTom20-GFP constructs carrying mutations in the COOH-terminal flanking region. Merged fluorescent images of GFP and MitoTracker are shown. (B) Fluorescent images of MitoTracker and rTom20-GFP mutants in which the basic amino acids in the flanking region were replaced by serine residues.