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. 2000 Oct 16;151(2):277–288. doi: 10.1083/jcb.151.2.277

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Proteasome inhibitor lactacystin does not affect the expression level and subcellular localization of rTom20-GFP constructs in COS-7 cells. (A) Effect of lactacystin on the expression of rTom20-GFP and H24GFP in COS-7 cells. COS-7 cells transfected with wild-type rTom20-GFP (WT) or H24GFP were grown at 37°C for 24 h, which were then incubated with or without 20 μM lactacystin (LC) for 10 h at 37°C. The cell lysates were subjected to SDS-PAGE followed by Western blotting with anti–rTom20 IgG. WT and H24 proteins are shown by an arrow. (B) Effect of lactacystin on the localization of H24GFP in COS-7 cells. H24GFP-expressed COS-7 cells prepared as in A were stained with MitoTracker. Merged confocal images of GFP (green) and MitoTracker (red) are shown.