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A three-color fluorescent assay of fusion. The cells were adhered to polylysine-coated glass. Effector cells are green (calcein) and target cells are purple (a mixture of the red DiI and the blue CMAC). Fused cells are pinkish. (A) Fusion did not occur at TAS. (B) After raising the temperature to 37°C for 30 min, fusion occurred and all three dyes redistributed (arrowheads).
