Table 1.

Primer sequences used for RT-PCR analysis

Target gene	Primer sequence 5′ → 3′	Location, nucleotide	Expected size, bp	Ref.
ERB
Set 1
ERβ-1	TGA AAA GGA AGG TTA GTG GGA ACC	230-253	528
ERβ-2	TGG TCA GGG ACA TCA TCA TGG	737-757
Set 2
ERβ-3	GCC CAA GAG AAG TGG CGG CCA CG	592-613	429	20
ERβ-4	AAA CCT TGA AGT AGT TGC CAG GAG C	996-1020
ERα
Set 1
ERα-1	TAC TGC ATC AGA TCC AAG GG	41-60	650	22
ERα-2	ATC AAT GGT GCA CTG GTT GG	671-690	(exon 1–3)
Set 2
ERα-3	GTG GGA ATG ATG AAA GGT GG	742-761	668
ERα-4	TCC AGA GAC TTC AGG GTG CT	1390-1409	(exon 3–6)
Set 3
ERα-5	GTG CCT GGC TAG AGA TCC TG	1142-1161	710
ERα-6	TGG TGC ATG ATG AGG GTA AA	1832-1851	(exon 5–8)
PR
PR1	GAT TCA GAA GCC AGC CAG AG	1817-1836	533
PR2	TGC CTC TCG CCT AGT TGA TT	2330-2349
AR
AR1	CTC TCT CAA GAG TTT GGA TGG CT	2896-2918	342
AR2	CAC TTG CAC AGA GAT GAT CTC TGC	3214-3237
GAPDH
GAPDH-F	CCA CCC ATG GCA AAT TCC ATG GCA	152-175	598	21
GAPDH-R	TCT AGA CGG CAG GTC AGG TCC ACC	726-749