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. 2008 Jan 15;22(2):152–165. doi: 10.1101/gad.1616208

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Figure 4. — Genetic characterization of R-NSCs and NSCs^FGF2/EGF. (A) Global gene expression analysis (Affymetrix, U133-Plus2) comparing Forse1⁺ versus Forse1⁻ R-NSCs at P1 (day 25). (B) Venn diagram representing genes specifically expressed in Forse1⁺ (red) and Forse1⁻ (green) R-NSCs, and in Forse1⁺ NSCs^FGF2/EGF (blue). R-NSC-specific genes are marked in yellow, while genes shared between all three NSC groups are marked in white. (C) Fold changes calculated from Affymetrix analysis for the top differentially expressed transcripts. Fold changes are grouped into those specific for R-NSCs, those shared between R-NSCs and NSCs^FGF2/EGF, and those specific to NSCs^FGF2/EGF. (D) Immunocytochemical confirmation of representative markers shared between R-NSCs and NSCs^FGF2/EGF (Zic1), or markers specific to R-NSC state (Dach1). (E) Colabeling studies of 3CB2 and NSCs^FGF2/EGF-specific (AQP4) or R-NSC-specific (PLZF) markers. Bar in D corresponds to 50 μm for all panels.