Skip to main content
. 2008 Jan 15;22(2):152–165. doi: 10.1101/gad.1616208

Figure 5.

Figure 5.

Effects of cell density, SHH, and Notch signaling pathways on R-NSC maintenance. (A) Effect of cell plating density and DAPT treatment on rosette reformation (Zic/ZO-1) and spontaneous neuronal differentiation (MAP2/Dcx) in dissociated P2 R-NSCs. Right panel shows quantification of rosette lumens as a surrogate marker of rosette growth. Statistical analysis: mean ± SEM; (***) P < 0.001; (**) P < 0.01 (compared with high density; ANOVA: Newman-Keuls test). (B) Phase contrast images of R-NSCs maintained in SHH/FGF8 over multiple passages (loss of rosette structure). (C) Schematic model illustrating symmetric versus asymmetric division mode in rosettes and relationship to lumen size. (D) Quantification of ZO-1+ rosette lumen size in P2 R-NSC cultures treated with various agonists and antagonists of candidate signaling pathways. Statistical analysis: mean ± SEM; (***) P < 0.001; (**) P < 0.01 (compared with control; ANOVA: Dunnett test). (E) Representative images (ZO-1/Sox1) of P2 R-NSC cultures at day 4 of treatment with candidate factors. Scale bar in A corresponds to 50 μm in A and B, and 75 μm in E.