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SCFCdc4 ubiquitylates PGC-1α in vivo. An in vivo ubiquitylation assay was performed by coexpressing the proteins indicated with HA-ubiquitin. (A) Whole-cell extracts (WCE; bottom panel) and Flag-PGC-1α immunoprecipitations (top two panels) were immunoblotted for the indicated proteins. (B) Flag-PGC-1α immunoprecipitated with anti-Flag antibody was immunoblotted with anti-Flag antibody.
