Figure 2.

Inhibition of endothelial-cell proliferation. Purified K1–5 at various concentrations was assayed on BCE cells in the presence of FGF-2 (1 ng/ml) in a 72-h proliferation experiment. (A) K1–5 displays a dose-dependent effect on suppression of BCE cell growth. (B and C) The maximal inhibitory activity was detected at 200 pM. Increase of concentrations does not enhance the inhibitory effect. (D) The inhibitory activity of K1–5 on BCE cells was reversible and endothelial cells regrew after removal of K1–5. Values represent the mean ± SEM of triplicate of each sample.