Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Feb 1;189(3):461–470. doi: 10.1084/jem.189.3.461

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Summary binding data for alanine-substituent mutants analyzed in this study. Free energy changes for F23.2 (top) and QL9/L^d (bottom) binding were calculated from competition experiments using IC_50(mutant)/IC_{50(wild-type)} and were normalized for KJ16/F23.1 reactivity to account for slight variations in sample refolding and purity. 1B2 reactivity (middle) was determined from a capture ELISA format, with the concentration of scTCR yielding 50% maximal signal used analogously to IC₅₀. ΔΔG values >0 indicate decreased binding.