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Production of IL-12 by DCs
| IL-12 p70 (pg/ml) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Untreated | Pansorbin + IFN-γ + GM-CSF | |||||||
| CD8α− | CD8α+ | CD8α− | CD8α+ | |||||
| Exp 1 | 11.5 | <8.0 | 13.8 | 190.1 | ||||
| Exp 2 | 12.7 | 11.1 | 33.3 | 812.2 | ||||
| Exp 3 | 17.7 | 19.8 | 93.3 | 854.8 | ||||
Low-density spleen cells were sorted according to CD8α expression and cultured with or without pansorbin plus IFN-γ plus GM-CSF. Supernatants were assayed for IL-12 p70 production 24 h later. Exp, experiment.
