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. 1999 Feb 1;189(3):575–586. doi: 10.1084/jem.189.3.575

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bad transgenic mice have decreased numbers of mature α/β T cells. (A) FACS^® analysis of CD4 vs. CD8 expression of thymocytes and (B) splenocytes from bad transgenic and nontransgenic littermates. Cells were stained with specific anti-CD4 and anti-CD8 antibodies. The subpopulations of CD8⁺CD4⁺, CD4⁺CD8⁻, and CD4⁻CD8⁺ T cells are gated and their percentages given. (C) Histogram indicating the percentage of TCR-γ/δ expressing cells within the CD4⁻CD8⁻ thymocytes of bad transgenic and nontransgenic littermates. The numbers in parentheses indicate the total number of TCR-γ/δ expressing cells in each thymus.

bad transgenic mice have decreased numbers of mature α/β T cells. (A) FACS^® analysis of CD4 vs. CD8 expression of thymocytes and (B) splenocytes from bad transgenic and nontransgenic littermates. Cells were stained with specific anti-CD4 and anti-CD8 antibodies. The subpopulations of CD8⁺CD4⁺, CD4⁺CD8⁻, and CD4⁻CD8⁺ T cells are gated and their percentages given. (C) Histogram indicating the percentage of TCR-γ/δ expressing cells within the CD4⁻CD8⁻ thymocytes of bad transgenic and nontransgenic littermates. The numbers in parentheses indicate the total number of TCR-γ/δ expressing cells in each thymus.