Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Feb 1;189(3):575–586. doi: 10.1084/jem.189.3.575

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Akt kinase activity in bad thymocytes is constitutively higher and is inhibited by wortmannin. Total cell lysates were prepared from thymocytes of bad transgenic mice and control littermates which had been cultured for up to 4 h. Akt was immunoprecipitated and an in vitro kinase assay was carried out using histone H2B as substrate. (A) The constitutive (0 h) level of kinase activity and the activity versus time of the nontransgenic control is shown in the left panel, and that of the bad transgenic mice in the right panel. (B) Akt kinase activity of bad transgenic thymocytes is shown in the absence (left) or presence (right) of 2 μM wortmannin over 4 h.

Akt kinase activity in bad thymocytes is constitutively higher and is inhibited by wortmannin. Total cell lysates were prepared from thymocytes of bad transgenic mice and control littermates which had been cultured for up to 4 h. Akt was immunoprecipitated and an in vitro kinase assay was carried out using histone H2B as substrate. (A) The constitutive (0 h) level of kinase activity and the activity versus time of the nontransgenic control is shown in the left panel, and that of the bad transgenic mice in the right panel. (B) Akt kinase activity of bad transgenic thymocytes is shown in the absence (left) or presence (right) of 2 μM wortmannin over 4 h.