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. 1999 Feb 1;189(3):493–500. doi: 10.1084/jem.189.3.493

Figure 1.

Figure 1

The mouse Ly-49D receptor, expressed on RNK-16 cells, mediates redirected lysis of the FcR-bearing target YB2/0 in the presence of anti–Ly-49D mAb. Staining for Ly-49D on wild-type RNK-16 cells (A) and RNK.mLy-49D transfectants (B) is shown by FACS® analysis of cells exposed to anti–Ly-49D mAb 12A8 (filled histogram) and FITC-conjugated goat anti–rat antibody (FITC-GAR) or FITC-GAR alone (dotted line). Standard 4-h cytotoxicity assays were performed with either FcR-bearing rat myeloma YB2/0 cells or YAC-1 cells as targets. RNK-16 effectors are shown on the left in C, E, and G, and RNK.mLy-49D effectors are shown on the right in D, F, and H. To measure redirected lysis by the Ly-49D receptor, effectors in C and D were preincubated with either media alone (□), intact anti–Ly-49D mAb (12A8) (♦), or intact isotype-matched control mAb (2C7) (○), before addition of YB2/0 targets. In E and F, effectors were preincubated with media alone (□), anti–Ly-49D F(ab′)2 fragments (▴), or control F(ab′)2 fragments (○). G and H show lysis of YAC-1 targets.