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. 1999 Feb 1;189(3):493–500. doi: 10.1084/jem.189.3.493

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The Ly-49D receptor activates lysis of H-2^d–expressing Con A blasts. RNK.mLy-49D transfectants were tested as effector cells for lysis of Con A blast targets prepared from mice that were congenic resistant at the H-2 locus. Con A blasts were prepared from splenocytes isolated from mice expressing either H-2^d (BALB/c, B10.D2; panels A and B), H-2^b (BALB.B, C57BL/6; panels C and D), or H-2^k (BALB.K, B10.BR; panels E and F). Assays were performed after preincubation of effector cells with media alone (□), anti–Ly-49D mAb (12A8) (♦), or control mAb (2C7) (○) before addition of targets.