Figure 3.
No effect on the growth rate of S194 and PD31 cells by E-T/2. (A) Growth curves. On day 0 (24 h after sorting), S194 and PD31 infected cells were plated at densities of 3 × 105 and 5 × 105 cells/ml, respectively in media containing 5% FCS. Viable cells were counted daily using a hemacytometer after trypan blue exclusion of the dead cells. The data is representative of several experiments. (B) Western blot using anti-E47 antibodies. Total cell extracts were prepared from S194 and PD31 cells infected with the indicated retrovirus. The E-T/2 and endogenous E2A proteins are indicated by the arrows. A degradation product of E-T/2 in PD31 cells is marked by an asterisk.