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. 1999 Feb 1;189(3):501–508. doi: 10.1084/jem.189.3.501

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Treatment of infected Jurkat/E cells with the caspase inhibitor, zVAD. (A) The growth of Jurkat/E cells infected with the vector or E-T/2 virus. Cells were sorted for GFP twice and plated in media containing 5% FCS without or with 1:500 vol/vol of DMSO or the same volume of zVAD (20 mM). On the indicated day after plating, cells were counted as previously described. On day 2, an equal volume of fresh medium containing the same concentration of zVAD or DMSO was added. Data shown are representatives of three separate experiments. (B) PI staining. On day 4 after plating, the cells cultured as in A were analyzed. The percentage of apoptotic cells is shown above the thin lines.

Treatment of infected Jurkat/E cells with the caspase inhibitor, zVAD. (A) The growth of Jurkat/E cells infected with the vector or E-T/2 virus. Cells were sorted for GFP twice and plated in media containing 5% FCS without or with 1:500 vol/vol of DMSO or the same volume of zVAD (20 mM). On the indicated day after plating, cells were counted as previously described. On day 2, an equal volume of fresh medium containing the same concentration of zVAD or DMSO was added. Data shown are representatives of three separate experiments. (B) PI staining. On day 4 after plating, the cells cultured as in A were analyzed. The percentage of apoptotic cells is shown above the thin lines.