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. 1999 Feb 15;189(4):747–752. doi: 10.1084/jem.189.4.747

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Binding of IgA mutants L1, L2, and L3 to purified human pIgR by ELISA. The extracellular domain of human pIgR was purified after expression in baculovirus and coated onto ELISA plates at 10 μg/ml. Chimeric IgA1 and IgA1 Cα3 mutants L1, L2, and L3 were expressed as both monomeric (m) and dimeric (d) forms along with chimeric IgG1, purified and incubated on the pIgR-coated plates to compare their abilities to bind to pIgR. Bound antibodies were detected with anti–human κ light chain alkaline phosphatase conjugate.