Table I.
New Polymorphic Markers Mapped to the Lps Region
| Locus | Probe | Repeat unit* | Primers 5′ → 3′ | Allele size | Polymorphism‡ | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| bp | ||||||||||
| P1-5227 | D4Mcg5 | [CT]23 [GT]23 | F: GGGAGGATGTAAAGCTTGAT | 141 | H>B=D>S | |||||
| R: CCAACATCCTCTTACCTCCA | ||||||||||
| P1-5226 | D4Mcg6 | [GT]24 | F: GCATAGGAACAGCTTCTGTGT | 122 | H=D>B | |||||
| R: TTCTATAAATAAGTACCT | ||||||||||
| Bac243o20 | D4Mcg10 | [CA]4TA[CA]25 | F: TCCTCAGTCACACTAGATTG | 120 | B=D>H>S | |||||
| R: TAGCCTGTGGTGTGATTAGTT | ||||||||||
| Yac89a3 | D4Mcg21 | [GT]19N22[AT]11 | F: CAGGGAGGCATATTCATA | 168 | H=D<B<S | |||||
| R: TCTAATTGCCTTTCAATC | ||||||||||
| Yac89a3 | D4Mcg24 | [GT]16 | F: GGTACCTCTATTAGGTGAATC | 113 | S>H>B=D | |||||
| R: ACAGTCTGACCTAGGTAGAGG | ||||||||||
| Yac89a3 | D4Mcg25 | [CA]24 | F: CCATGCTGGGAATCTCACAAC | 164 | D<H=B<S | |||||
| R: CTATTCTAGAGCCAGTCATTTC | ||||||||||
| Yac89a3 | D4Mcg27 | [GT]25 | F: CTTTCATTAGACAGATTATAT | 140 | H>B=D>S | |||||
| R: CAGACAGTATGAACTCCTG |
*
The base composition of each simple sequence repeat is indicated. A, adenine; C, cytosine; G, guanine; T, thymidine. Numeric subscripts denote the length of each dinucleotide repeat shown in brackets. N22 = 22-bp nonrepetitive sequence.
‡
Relative allele sizes among inbred mouse strains as generated by PCR using flanking forward (F) and reverse (R) primers. B, C57BL/6J; D, DBA/ 2J; H, C3H/HeJ; S, M. spretus.