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. 1999 Mar 1;189(5):811–820. doi: 10.1084/jem.189.5.811

Figure 1.

Figure 1

Expression of unmutated tumor antigen. (a) Expression of P1A RNA in normal tissues. Total RNA was isolated from liver (Li), thymus (Th), spleen (Sp), lung (Lu), and testis (Te) of normal BALB/c mice, and the expression of the P1A gene was determined by RT-PCR. The primers used detect a 0.5-kb fragment from mature P1A RNA but a 3.1-kb fragment from the genomic DNA. GAPDH primers were used as a control for the amount of cDNA used in each sample. The sources of RNA for different lanes are indicated at the bottom. Note that the autoradiograph of the left four lanes was exposed for 4 h, whereas that of the right two lanes was exposed for 10 min. (b) Comparison of the amounts of P1A RNA in normal spleens and J558 cells. First strand cDNA prepared from 5 μg of total RNA from either normal spleen or J558 cells was suspended in 50 μl of water. 1, 0.1, 0.01, or 0.001 μl of the cDNA was used as a template for PCR reaction using either P1A or GAPDH primers as described in Materials and Methods. 20 μl of PCR products was separated in agarose gels and the amount of specific products detected by Southern blot.