Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Mar 1;189(5):811–820. doi: 10.1084/jem.189.5.811

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Clonal deletion of P1A-specific transgenic T cells in mice overexpressing P1A in the thymus. (a) Overexpression of P1A mRNA in the thymus of P1A-transgenic mice (P1A-TG) as compared to that of nontransgenic mice (Non-TG). cDNA, prepared from total mRNA of J558 tumor cells, thymus P1A-TG, and Non-TG thymus, as described in the Fig. 1 legend, were diluted 1:10, 1:30, 1:90, or 1:270. 1 μl of the cDNA from each dilution was used for the PCR reaction. PCR products were transferred into nylon membrane, and the identity of the PCR products verified by ³²P-labeled probes. The PCR products were not amplified from genomic DNA, as no P1A can be amplified in the absence of RT (data not shown). (b) Intrathymic clonal deletion of transgenic T cells in mice overexpressing tumor antigen in the thymus. Thymocytes (top) and spleen cells (bottom) from either TCR–TG or TCR/P1A–TG mice were analyzed by two-color flow cytometry for expression of CD4 vs. CD8 or CD8 vs. Vα8.

Clonal deletion of P1A-specific transgenic T cells in mice overexpressing P1A in the thymus. (a) Overexpression of P1A mRNA in the thymus of P1A-transgenic mice (P1A-TG) as compared to that of nontransgenic mice (Non-TG). cDNA, prepared from total mRNA of J558 tumor cells, thymus P1A-TG, and Non-TG thymus, as described in the Fig. 1 legend, were diluted 1:10, 1:30, 1:90, or 1:270. 1 μl of the cDNA from each dilution was used for the PCR reaction. PCR products were transferred into nylon membrane, and the identity of the PCR products verified by ³²P-labeled probes. The PCR products were not amplified from genomic DNA, as no P1A can be amplified in the absence of RT (data not shown). (b) Intrathymic clonal deletion of transgenic T cells in mice overexpressing tumor antigen in the thymus. Thymocytes (top) and spleen cells (bottom) from either TCR–TG or TCR/P1A–TG mice were analyzed by two-color flow cytometry for expression of CD4 vs. CD8 or CD8 vs. Vα8.