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. 1999 Mar 1;189(5):821–829. doi: 10.1084/jem.189.5.821

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Increase in MHC class I biosynthesis and stability induced by PR8 infection allows efficient presentation of viral antigen to cytotoxic T cells. (A) Synthetic rate and stability of HLA class I molecules in immature DCs and in DCs that were stimulated for 5 h with 5 HAU/ml PR8 or LPS. Labeled class I molecules were precipitated after 1 h of chase (time 0) or after 12 or 24 h. (B) Half-life of labeled HLA class I molecules quantitated by PhosphorImager in immature DCs (○), LPS-treated DC (•), or PR8-infected DC (▴). (C) Proliferative response of an HLA-A2– restricted M58-66–specific T cell clone cultured with graded numbers of HLA-A2⁺ DCs. DCs were either pulsed with 1 μM M58-66 peptide (circles) or infected with 5 HAU/ml PR8 with (triangles) or without (squares) IFN-α (500 U/ml) pretreatment. DCs were tested 5 h (open symbols) or 24 h after pulsing (filled symbols).