Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Mar 1;189(5):831–841. doi: 10.1084/jem.189.5.831

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

After affinity purification, anti-Nramp1 and anti-Nramp2 antisera were tested for specificity and reactivity by immunoblotting against crude membrane fractions (A) as well as by immunoprecipitation of [³⁵S]methionine–labeled cell lysates (B) from CHO cells or from the same cells transfected with a c-myc–tagged (tag sequence EQKLISEEDL) Nramp1 (CHON1) or a c-myc–tagged Nramp2 (CHON2). Protein extracts were separated by SDS-PAGE and either transferred to membranes (A) or exposed to x-ray films after immunoprecipitation (B). Antisera directed against Nramp2 (top), Nramp1 (center) or a commercially available mouse monoclonal anti–c-myc antibody (9E10; bottom) were used. The size of the molecular mass markers (in kD) is indicated to the right of the gels.