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. 1999 Mar 1;189(5):831–841. doi: 10.1084/jem.189.5.831

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Subcellular localization of Nramp1 and Nramp2 in transfected CHO cells. Immunofluorescence was performed on CHO cells transfected with c-myc–tagged Nramp1 proteins (A and B), c-myc–tagged Nramp2 proteins (C and D), or with untransfected CHO controls (E and F). Immunofluorescence was with the anti–c-myc mouse monoclonal antibody 9E10 (A, C, and E) which detects the transfected Nramp1 and Nramp2 proteins. Lysosomes were labeled by preincubation of the cells with FITC–conjugated dextran (B, D, and F). Cells were then fixed and reacted with the anti–c-myc tag antibody 9E10 and a rhodamine-conjugated secondary antibody (A, C, and E), followed by examination by fluorescence microscopy and photography.