Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Mar 1;189(5):831–841. doi: 10.1084/jem.189.5.831

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Nramp2 association with phagosomes. (A) Immunoblotting of latex bead–containing phagosomes isolated from J77a cells. Latex bead–containing phagosomes were purified from cell homogenates by subcellular fractionation on sucrose density gradients as described in Materials and Methods. Equal amounts of phagosomal proteins (P) and of a crude membrane protein extract prepared prior to phagocytosis (M) were separated by SDS-PAGE on a 7.5% gel. Proteins were transferred to nitrocellulose and the immunoblot was sequentially analyzed with anti-Nramp2 antiserum (left), anti-Lamp1 antibody (center), and anti-transferrin receptor antibody (right). The position of molecular mass markers (in kD) is indicated on the left side of the immunoblot. (B) Localization of the Nramp2 and Lamp1 proteins in J774a macrophages by immunofluorescence. J774a macrophages were allowed to phagocytose latex beads, then fixed and stained with the anti-Nramp2 antiserum and a rhodamine-conjugated secondary antibody (a), and the anti-Lamp1 antibody and a secondary antibody coupled to FITC (b). A phase contrast image of the cells shown in panels a and b is also included in panel c.

Nramp2 association with phagosomes. (A) Immunoblotting of latex bead–containing phagosomes isolated from J77a cells. Latex bead–containing phagosomes were purified from cell homogenates by subcellular fractionation on sucrose density gradients as described in Materials and Methods. Equal amounts of phagosomal proteins (P) and of a crude membrane protein extract prepared prior to phagocytosis (M) were separated by SDS-PAGE on a 7.5% gel. Proteins were transferred to nitrocellulose and the immunoblot was sequentially analyzed with anti-Nramp2 antiserum (left), anti-Lamp1 antibody (center), and anti-transferrin receptor antibody (right). The position of molecular mass markers (in kD) is indicated on the left side of the immunoblot. (B) Localization of the Nramp2 and Lamp1 proteins in J774a macrophages by immunofluorescence. J774a macrophages were allowed to phagocytose latex beads, then fixed and stained with the anti-Nramp2 antiserum and a rhodamine-conjugated secondary antibody (a), and the anti-Lamp1 antibody and a secondary antibody coupled to FITC (b). A phase contrast image of the cells shown in panels a and b is also included in panel c.