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. 1999 Mar 1;189(5):767–778. doi: 10.1084/jem.189.5.767

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Production of TNF by CD4⁺ T cells stimulated with autologous EBV-B cells incubated with protein MAGE-3. EBV-B cells from hemochromatosis patient LB 1555 were incubated with 20 μg/ml of OVA or MAGE-3 protein for 20 h. 5,000 cells were distributed in microwells to which an aliquot of each of the 96 CD4 microcultures was added (∼3,000 effector cells). TNF production was estimated after overnight culture, by the toxicity of the supernatants for the TNF-sensitive WEHI 164-13 cells. The dots represent the average result obtained with two aliquots of each CD4 microculture.